量子生物科技

新一代 SuperScript® IV Reverse Transcriptase

別讓樣本征服了您的實驗!!

新一代的 SuperScript® IV Reverse Transcriptase 具備了

◉ 對 RNA 樣本中的抑制物具高抗性
◉ 更高的熱穩定性(可達55℃)
◉ 適用的 RNA 樣本來源更廣
◉ 反應時間更快,只需10分鐘


RNA 樣本中的抑制物對 SuperScript® IV Reverse Transcriptase 影響小

RNA sample中的抑制物

InhibitorSource
Ethanol/isopropanol, Salts, Phenol/chloroform, DetergentsSample prep
Heparin, Hematin, Bile saltsBlood, Feces
Humic acid, Polyphenols, PolysaccharidesSoil, Plants
Formalin, ParaffinFFPE

No
inhibitors

Biological inhibitors
0.01 U/ul Heparin

Sample Prep inhibitors
5% Ethanol

Figure1. Analysis of first-strand cDNA synthesis performed in the presence of both biological and sample prep inhibitors.


針對降解的RNA樣本,以及難反轉錄的RNA樣本來源,具有高敏感性和cDNA合成可重複性的優勢。

Figure2. Higher reverse transcription efficiency with degraded RNA using SuperScript® IV RT.


最低只需10分鐘即可合成cDNA,且不影響產率。

Figure3. Fast cDNA synthesis rate in just 10 minutes.

Figure4. The cDNA synthesis protocol for SuperScript® IV Reverse Transcriptase


訂購資訊

品名包裝編號
SuperScript® IV Reverse Transcriptase2,000 units18090010
SuperScript® IV Reverse Transcriptase10,000 units18090050
SuperScript® IV First-Strand Synthesis System50 rxns18091050
SuperScript® IV First-Strand Synthesis System200 rxns18091200

獲得更多訊息可詢問量子生物科技區域業務專員。

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